human epithelial cell line a 375  (ATCC)

Journal: Cancers

Article Title: The In Vitro and In Vivo Anticancer Properties of Chalcone Flavokawain B through Induction of ROS-Mediated Apoptotic and Autophagic Cell Death in Human Melanoma Cells

doi: 10.3390/cancers12102936

Figure Lengend Snippet: Flavokawain B (FKB) inhibited the growth of human melanoma cells. ( A – D ) human epithelial melanoma cell line A375, human skin lymph node derived melanoma cell line A2058, human primary epidermal melanocyte cell line (HEMn), and human skin keratinocyte cell line (HaCaT) cells were exposed to 0–20 µg/mL concentrations of FKB for 24 h. Then, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was carried out to determine cell viability. The IC 50 value for each cell type was determined as described in the methods section (inset: IC 50 values of respective cell lines). ( E ) FKB mediated morphological changes in A375 and A2058 cells were examined using the phase-contrast microscope. ( F ) Expression patterns of BRAF and p-ERK proteins were determined by Western blot to discover the effect of FKB concentrations (0–10 μg/mL) for 24 h. β-actin was used as an internal control. Each value was expressed as mean ± standard deviation of three experiments. Statistical significance was assigned as ** p < 0.01 and *** p < 0.001 as compared to the untreated control cells.

Article Snippet: Human epithelial melanoma cell line (A375), human skin lymph node derived melanoma cell line (A2058 [ ]), human primary epidermal melanocyte cell line (HEMn), and human skin keratinocyte cell line (HaCaT) cells were purchased from American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Derivative Assay, MTT Assay, Microscopy, Expressing, Western Blot, Control, Standard Deviation